Ali Karami

ABSTRACT: DNA vaccine have many potential advantage over traditional vaccine. There is no risk of infection , its superior to protein or polysaccharides and recombinant vaccine because DNA is very stable, its production is easy and most suitable for the use in developing countries. But most exciting finding about advantage of DNA vaccine is its ability to induce both cellular and humoral response, it works like inactivated live viral vaccines. Several experiment have shown immunogenicity of Nucleic Acid immunization with HBs-Ag encoding plasmid. We have studied the expression of HBs antigen and anti HBs response in mice by intramuscular and intradermal injection of recombinant eukaryotic vector encoding hepatitis B surface antigen sequence next to CMV promoter. ELISA analysis of serum samples from mice injected with 50 or 100 ug of endotoxin free DNA with and without special new polymer synthetized in IRAN (dendrosome) revealed that : small amount of HBsAg expressed in mice tissue can strongly stimulate immune response and high level of anti-HBs was detected in both endotoxin freeDNA alone and with polymer but Dendrosome mediated DNA vaccination has elevetated antibody production comparing to non adjuvant DNA. Enhanced immune response was detected as 10 fold comparing to four times administration of human recombinant (protein) HBs vaccine. PCR analysis of blood and muscle tissue injection with DNA vaccine for presence of HBs sequence in different time intervals revealed that after 72hr we were not able to detect from HBs-Ag encoding sequence.
DNA vaccine