Ramezan Ali Ataee, mahmod Ghorbani, Mahdy Kamali, Reza Ranjbar, Ali Karami

ABSTRACT: Abstract Aim: The aim of this study was to standardize the molecular detection Staphylococcus aureus enterotoxin C gene method. Materials and Methods: Based on gene bank data, the PCR molecular method was set up for detection of entC gene in Staphylococcus aureus isolated from human subjects (300 isolated strains were assayed). The PCR Product was sequenced and were compared with the standard gene. By using the ELISA test, ability to produce enterotoxin C of all strains which hair born entC gene were evaluated and analyzed by ELISA kit. Results: The results showed that the molecular technique of PCR is well set up. Because, the first primer pair amplified a 102bp fragment and the second primer pair were amplified a 1223bp fragment with ease. Multiple Alignment the sequence of 1223bp fragment with the reference gene showed 99 percent compliance. They translated form shows only difference in position 218 which the alanine was replace the valine. Using ELISA test the toxigenicity of all strain containing entC gene was shown that the 37% of strains contained the entC gene. Conclusion: in addition of coagulase positive Staphylococcus aureus strains coagulase negative strains have ability to produce supperantigenic enterotoxins. This research provides a simple and quick confirmation and identification method of which capable detect enterotoxin C-producing strains and it has been standardized. Because of the diagnosis the supperantigens could represent valuable guide for proper treatment and prevention of complications of them.

Journal of Military Medicine. 01/2013