دکتر رمضانعلی عطایی 1*، دکتر علی مهرابی توانا 2، دکتر علی کرم ی 2، دکتر مرتضی ایزد ی 3، دکتر سیدمحمدجواد حسن ی 2
زهرا سفیری 2، محمد الله وردی 1
-1 گروه میکروب شناسی، مرکز تحقیقات کاربرد درمانی توکسین های می کروبی، دانشگاه علوم پزشکی بقیه ا... 2- مرکز تحقیقات بیولوژی مولکولی، دانشکده پزشکی،
دانشگاه علوم پزشکی بقی ها... 3- مرکز تحقیقات بهداشت، دانشکده پزشکی، دانشگاه علوم پزشکی بقیه ا...

Title: The Assessment of Universal Primer for Rapid Detection of Bacterial Meningitis

Authors: Ataee RA, (PhD); Mehrabi Tavana A, (PhD); Karami A, (PhD); Izadi M, (MD); Hossaini
SMJ, (MD); Safiri Z, (MSc); Aalahverdi M, (MSc).

Introduction: The aim of this study was to design a molecular detection for differentiation of bacterial
from nonbacterial meningitis.
Methods: In this research, the universal primer was used. The DNA of 20 bacterial strains was
extracted. Eubacterial 16SrRNA gene fragments (16S rDNA) were amplified by PCR with broad-range
ribosomal primers. The PCR was curried out under optimized standard conditions. The PCR products
were electrophoresed on agarose 1 % and compared with standard strains. The high specificity of the PCR
assay was documented after testing bacteria of 20 different species.
Results: At least 10 bacteria could be found in 1 ml of fluid culture. The use of universal primer which
contains 16SrRNA could be suitable amplification of a sequence with 1000bp. This sequence was found
very similar in nearly all bacteria. Twenty different bacterial strains were tested. The sequence (1000bp)
was seen in all bacterial strains applied in this study.
Conclusion: Use of this molecular method for any bacterial meningitis is applicable because of fast
diagnosis in 3 hours, differentiating bacterial meningitis from non-bacterial, and finally the effective
treatment of patients suffered with the disease. The method is effective in developing and developed
countries.
Keywords: Bacterial meningitis, diagnosis, primer 16S rRNA, PCR.
Hakim Research Journal 2009; 11(4): 27