R Ranjbar 1, MR Pourshafie 2, N Sadeghifard 3, A Karami1, M Hamidian 2, M Izadi 4,
M Parzadeh 2, N Jonaidi 4
1Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
2Dept. of Bacteriology, Pasteur Institute of Iran, Tehran, Iran
3 Dept. of Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran
4Health Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
(Received 29 Sep 2007; accepted 18 Mar 2008)

Abstract
Background: Epidemic and endemic cholera is a major public health problem for many countries. Aim of this study was
to evaluate AP-PCR for investigation of clonal relatedness among the strains of Vibrio cholerae recovered from an outbreak
occurred in different parts of Iran in 2005.
Methods: The study was conducted during the cholera outbreak occurred in some of provinces in Iran in summer 2005.
Bacterial isolation and identification was carried out according to the standard bacteriological methods. Arbitrarily primed
PCR (AP-PCR) used to study the genetic relatedness between the V.cholerae isolates.
Results: Thirty-nine isolates of V.cholerae O1 were identified. All isolates belonged to serotype Inaba. AP-PCR could differentiate
the isolates into five groups. AP-PCR cluster types 1 and 2 were the most prevalent groups, accounting for 36%
and 41%, respectively, of V.cholerae isolates.
Conclusion: The most of epidemic strains of V.cholerae O1 isolated in the year 2005 could be attributed to two predominant
clusters including AP-PCR cluster types 1 and 2 accounting for more than 77% of isolates. In conclusion, a few
epidemic clones were responsible for the apparently epidemic occurrence of cholera in provinces studied.
Keywords: Vibrio cholerae O1, AP-PCR, Molecular epidemiology